› 4l8f

X-ray diffraction
1.97Å resolution

Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX

Released:
DOI: 10.2210/pdb4l8f/pdb
PDB entry 4l8f coloured by chain, front view.
PDB entry 4l8f coloured by chain, side view.
PDB entry 4l8f coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish γ-glutamyl hydrolase.
Chuankhayan P, Kao TT, Lin CC, Guan HH, Nakagawa A, Fu TF, Chen CJ
J Med Chem 56 7625-35 (2013)
PMID: 24028568

Function and Biology Details

Reaction catalysed: 
Tetrahydropteroyl-(gamma-glutamyl)(n) + (n-1) H(2)O = 5,6,7,8-tetrahydrofolate + (n-1) L-glutamate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
folate gamma-glutamyl hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 312 amino acids
Theoretical weight: 35.38 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NY42 (Residues: 1-312; Coverage: 100%)
Gene names: ggh, wu:fa56a05, wu:fj90a01, zgc:76958
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MTX 2 x MTX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1, NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 53.396Å b: 198.098Å c: 65.112Å
α: 90° β: 113.816° γ: 90°
R-values:
R R work R free
0.178 0.175 0.239
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Functional analysis of Rossmann-like domains reveals convergent evolution of topology and reaction pathways.
Medvedev et al. (2019)
1 more