› 4l98

X-ray diffraction
2.28Å resolution

Crystal structure of the complex of F360L PPARgamma mutant with the ligand LT175

Released:
DOI: 10.2210/pdb4l98/pdb
PDB entry 4l98 coloured by chain, front view.
PDB entry 4l98 coloured by chain, side view.
PDB entry 4l98 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the transactivation deficiency of the human PPARγ F360L mutant associated with familial partial lipodystrophy.
Lori C, Pasquo A, Montanari R, Capelli D, Consalvi V, Chiaraluce R, Cervoni L, Loiodice F, Laghezza A, Aschi M, Giorgi A, Pochetti G
Acta Crystallogr D Biol Crystallogr 70 1965-76 (2014)
PMID: 25004973

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome proliferator-activated receptor gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 31.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 235-505; Coverage: 54%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand LRG 2 x LRG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 64.41Å b: 112.46Å c: 117.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural and physico-chemical effects of disease and non-disease nsSNPs on proteins.
Kucukkal et al. (2015)
6 other articles

1 mention without citation

Structural dataset for the PPARγ V290M mutant.
Puhl et al. (2016)