› 4lgi

X-ray diffraction
2.3Å resolution

N-terminal truncated NleC structure

Released:
DOI: 10.2210/pdb4lgi/pdb
PDB entry 4lgi coloured by chain, front view.
PDB entry 4lgi coloured by chain, side view.
PDB entry 4lgi coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Structure and mechanism of a type III secretion protease, NleC.
Li W, Liu Y, Sheng X, Yin P, Hu F, Liu Y, Chen C, Li Q, Yan C, Wang J
Acta Crystallogr D Biol Crystallogr 70 40-7 (2014)
PMID: 24419377

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186855 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
T3SS secreted effector NleC Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 30.11 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8X834 (Residues: 56-319; Coverage: 78%)
Gene names: ECs_0847, nleC
Sequence domains: NFkB-p65-degrading zinc protease

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 59.512Å b: 88.664Å c: 110.653Å
α: 90° β: 92.89° γ: 90°
R-values:
R R work R free
0.254 0.253 0.28
Expression system: Escherichia coli

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Citations

5 review citations

Bringing down the host: enteropathogenic and enterohaemorrhagic Escherichia coli effector-mediated subversion of host innate immune pathways.
Santos et al. (2015)
4 more