› 4lhk

X-ray diffraction
1.73Å resolution

Structure of the N-terminal domain of the Lg-Flo1 adhesin (N-Lg-Flo1p) from the yeast Saccharomyces pastorianus, in complex with calcium and alpha-1,2-mannobiose

Released:
DOI: 10.2210/pdb4lhk/pdb
Model geometry
Fit model/data
PDB entry 4lhk coloured by chain, front view.
PDB entry 4lhk coloured by chain, side view.
PDB entry 4lhk coloured by chain, top view.
Source organism: Saccharomyces pastorianus
Primary publication:
Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival.
Goossens KV, Ielasi FS, Nookaew I, Stals I, Alonso-Sarduy L, Daenen L, Van Mulders SE, Stassen C, van Eijsden RG, Siewers V, Delvaux FR, Kasas S, Nielsen J, Devreese B, Willaert RG
OpenAccess logo mBio 6 (2015)
PMID: 25873380

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
PA14 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 26.42 KDa
Source organism: Saccharomyces pastorianus
Expression system: Escherichia coli
UniProt:
  • Canonical: B3IUB3 (Residues: 23-213; Coverage: 100%)
Gene name: Lg-FLO1
Sequence domains: PA14 domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 38.618Å b: 85.476Å c: 68.24Å
α: 90° β: 93.72° γ: 90°
R-values:
R R work R free
0.14 0.137 0.193
Expression system: Escherichia coli

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Citations

9 review citations

What We Do Not Know about Fungal Cell Adhesion Molecules.
Lipke PN. (2018)
8 more

4 mentions without citation

Adhesins of Yeasts: Protein Structure and Interactions.
Willaert RG. (2018)
3 more