Function and Biology Details
Reaction catalysed:
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Glycosyl hydrolase, family 13, catalytic domain
- 1,4-alpha-glucan-branching enzyme, GlgB
- Alpha-amylase/branching enzyme, C-terminal all beta
- Glycosyl hydrolase, all-beta
- Glycoside hydrolase superfamily
- Glycoside hydrolase, family 13, N-terminal
- Glycogen branching enzyme GlgB, N-terminal Early set domain
- 1,4-alpha-glucan-branching enzyme
2 more domains
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
1,4-alpha-glucan branching enzyme GlgB (preferred)
PDBe Complex ID:
PDB-CPX-139746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
1,4-alpha-glucan branching enzyme GlgB
Molecule details ›
Chains: A, B, C, D
Length: 612 amino acids
Theoretical weight: 71.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 612 amino acids
Theoretical weight: 71.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P07762 (Residues: 117-728; Coverage: 84%)
Sequence domains:
- Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
- Alpha amylase, catalytic domain
- Alpha amylase, C-terminal all-beta domain
Ligands and Environments
Experiments and Validation Details
X-ray source:
APS BEAMLINE 21-ID-D
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)
