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Structural changes during cysteine desulfurase CsdA and sulfur acceptor CsdE interactions provide insight into the trans-persulfuration.

Kim S, Park S
J Biol Chem 288 27172-27180 (2013)
Cited: 28 times
EuropePMC logo PMID: 23913692

Abstract

In Escherichia coli, three cysteine desulfurases (IscS, SufS, and CsdA) initiate the delivery of sulfur for various biological processes such as the biogenesis of Fe-S clusters. The sulfur generated as persulfide on a cysteine residue of cysteine desulfurases is further transferred to Fe-S scaffolds (e.g. IscU) or to intermediate cysteine-containing sulfur acceptors (e.g. TusA, SufE, and CsdE) prior to its utilization. Here, we report the structures of CsdA and the CsdA-CsdE complex, which provide insight into the sulfur transfer mediated by the trans-persulfuration reaction. Analysis of the structures indicates that the conformational flexibility of the active cysteine loop in CsdE is essential for accepting the persulfide from the cysteine of CsdA. Additionally, CsdA and CsdE invoke a different binding mode than those of previously reported cysteine desulfurase (IscS) and sulfur acceptors (TusA and IscU). Moreover, the conservation of interaction-mediating residues between CsdA/SufS and CsdE/SufE further suggests that the SufS-SufE interface likely resembles that of CsdA and CsdE.

Articles - 4lw4 mentioned but not cited (12)

  1. Updates to the Integrated Protein-Protein Interaction Benchmarks: Docking Benchmark Version 5 and Affinity Benchmark Version 2. Vreven T, Moal IH, Vangone A, Pierce BG, Kastritis PL, Torchala M, Chaleil R, Jiménez-García B, Bates PA, Fernandez-Recio J, Bonvin AM, Weng Z. J Mol Biol 427 3031-3041 (2015)
  2. Structural changes during cysteine desulfurase CsdA and sulfur acceptor CsdE interactions provide insight into the trans-persulfuration. Kim S, Park S. J Biol Chem 288 27172-27180 (2013)
  3. Escherichia coli SufE sulfur transfer protein modulates the SufS cysteine desulfurase through allosteric conformational dynamics. Singh H, Dai Y, Outten FW, Busenlehner LS. J Biol Chem 288 36189-36200 (2013)
  4. Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS. Dunkle JA, Bruno MR, Outten FW, Frantom PA. Biochemistry 58 687-696 (2019)
  5. SufE D74R Substitution Alters Active Site Loop Dynamics To Further Enhance SufE Interaction with the SufS Cysteine Desulfurase. Dai Y, Kim D, Dong G, Busenlehner LS, Frantom PA, Outten FW. Biochemistry 54 4824-4833 (2015)
  6. Changes in Protein Dynamics in Escherichia coli SufS Reveal a Possible Conserved Regulatory Mechanism in Type II Cysteine Desulfurase Systems. Kim D, Singh H, Dai Y, Dong G, Busenlehner LS, Outten FW, Frantom PA. Biochemistry 57 5210-5217 (2018)
  7. X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Roret T, Pégeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C. Acta Crystallogr F Struct Biol Commun 70 1180-1185 (2014)
  8. Enhanced sampling of protein conformational states for dynamic cross-docking within the protein-protein docking server SwarmDock. Torchala M, Gerguri T, Chaleil RAG, Gordon P, Russell F, Keshani M, Bates PA. Proteins 88 962-972 (2020)
  9. Structural diversity of cysteine desulfurases involved in iron-sulfur cluster biosynthesis. Fujishiro T, Nakamura R, Kunichika K, Takahashi Y. Biophys Physicobiol 19 1-18 (2022)
  10. Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases. Dunkle JA, Bruno MR, Frantom PA. Acta Crystallogr D Struct Biol 76 291-301 (2020)
  11. Insights into the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE. Peña-Soler E, Aranda J, López-Estepa M, Gómez S, Garces F, Coll M, Fernández FJ, Tuñon I, Vega MC. PLoS One 12 e0186286 (2017)
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Reviews citing this publication (6)

  1. Recent advances in the Suf Fe-S cluster biogenesis pathway: Beyond the Proteobacteria. Outten FW. Biochim Biophys Acta 1853 1464-1469 (2015)
  2. Hydrogen Sulfide and Persulfides Oxidation by Biologically Relevant Oxidizing Species. Benchoam D, Cuevasanta E, Möller MN, Alvarez B. Antioxidants (Basel) 8 E48 (2019)
  3. Fe-S cluster biogenesis by the bacterial Suf pathway. Blahut M, Sanchez E, Fisher CE, Outten FW. Biochim Biophys Acta Mol Cell Res 1867 118829 (2020)
  4. Assembly and Transfer of Iron-Sulfur Clusters in the Plastid. Lu Y. Front Plant Sci 9 336 (2018)
  5. Iron-sulfur clusters biogenesis by the SUF machinery: close to the molecular mechanism understanding. Pérard J, Ollagnier de Choudens S. J Biol Inorg Chem 23 581-596 (2018)
  6. Possible molecular basis of the biochemical effects of cysteine-derived persulfides. Cuevasanta E, Benchoam D, Semelak JA, Möller MN, Zeida A, Trujillo M, Alvarez B, Estrin DA. Front Mol Biosci 9 975988 (2022)

Articles citing this publication (10)



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