› 4lw9

X-ray diffraction
1.9Å resolution

Crystal structure of Vibrio cholera major pseudopilin EpsG

Released:
DOI: 10.2210/pdb4lw9/pdb
PDB entry 4lw9 coloured by chain, front view.
PDB entry 4lw9 coloured by chain, side view.
PDB entry 4lw9 coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Vago FS, Raghunathan K, Jens JC, Wedemeyer WJ, Bagdasarian M, Brunzelle JS, Arvidson DN

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system core protein G Chains: A, B, C, D, E, F, I, J, K, L, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, I, J, K, L, Q, R
Length: 120 amino acids
Theoretical weight: 13.39 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45773 (Residues: 35-146; Coverage: 77%)
Gene names: VC_2730, epsG
Sequence domains: Type II secretion system (T2SS), protein G
Structure domains: Glycoprotein, Type 4 Pilin

Ligands and Environments

6 bound ligands:
Ligand ZN 32 x ZN
Ligand NA 6 x NA
Ligand CA 14 x CA
Ligand CL 9 x CL
Ligand PT 12 x PT
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 88.61Å b: 70.015Å c: 131.544Å
α: 90° β: 103.29° γ: 90°
R-values:
R R work R free
0.205 0.202 0.245
Expression system: Escherichia coli BL21(DE3)

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