› 4m0k

X-ray diffraction
1.4Å resolution

Crystal structure of adenine phosphoribosyltransferase from Rhodothermus marinus DSM 4252, NYSGRC Target 029775.

Released:
DOI: 10.2210/pdb4m0k/pdb
PDB entry 4m0k coloured by chain, front view.
PDB entry 4m0k coloured by chain, side view.
PDB entry 4m0k coloured by chain, top view.
Source organism: Rhodothermus marinus DSM 4252
Entry authors: Malashkevich VN, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112027 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 199 amino acids
Theoretical weight: 22.1 KDa
Source organism: Rhodothermus marinus DSM 4252
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0MGF4 (Residues: 1-177; Coverage: 100%)
Gene names: Rmar_0813, apt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand AMP 4 x AMP
Ligand CA 4 x CA
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 40.214Å b: 64.609Å c: 73.148Å
α: 87.3° β: 77.23° γ: 85.37°
R-values:
R R work R free
0.185 0.184 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links