› 4m18

X-ray diffraction
3.2Å resolution

Crystal Structure of Surfactant Protein-D D325A/R343V mutant in complex with trimannose (Man-a1,2Man-a1,2Man)

Released:
DOI: 10.2210/pdb4m18/pdb
PDB entry 4m18 coloured by chain, front view.
PDB entry 4m18 coloured by chain, side view.
PDB entry 4m18 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanisms of inhibition of influenza by surfactant protein D revealed by large-scale molecular dynamics simulation.
Goh BC, Rynkiewicz MJ, Cafarella TR, White MR, Hartshorn KL, Allen K, Crouch EC, Calin O, Seeberger PH, Schulten K, Seaton BA
Biochemistry 52 8527-38 (2013)
PMID: 24224757

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Pulmonary surfactant-associated protein D Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 147 amino acids
Theoretical weight: 15.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P35247 (Residues: 229-375; Coverage: 41%)
Gene names: COLEC7, PSPD, SFTP4, SFTPD
Sequence domains:
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
1 bound ligand:
Ligand CA 38 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 107.833Å b: 159.454Å c: 159.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.222
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Playing hide and seek: how glycosylation of the influenza virus hemagglutinin can modulate the immune response to infection.
Tate et al. (2014)
11 more

3 mentions without citation

The phosphoproteome of bloodstream form Trypanosoma brucei, causative agent of African sleeping sickness.
Nett et al. (2009)
2 more