› 4m3k

X-ray diffraction
1.48Å resolution

Structure of a single domain camelid antibody fragment cAb-H7S in complex with the BlaP beta-lactamase from Bacillus licheniformis

Released:
DOI: 10.2210/pdb4m3k/pdb
PDB entry 4m3k coloured by chain, front view.
PDB entry 4m3k coloured by chain, side view.
PDB entry 4m3k coloured by chain, top view.
Source organisms:
Entry authors: Pain C, Kerff F, Herman R, Sauvage E, Preumont S, Charlier P, Dumoulin M

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208316 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.41 KDa
Source organism: Bacillus licheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P00808 (Residues: 44-211, 212-307; Coverage: 94%)
Gene names: blaP, penP
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily
Camelid heavy-chain antibody variable fragment cAb-H7S Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 13.52 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 51.818Å b: 42.94Å c: 74.818Å
α: 90° β: 105.29° γ: 90°
R-values:
R R work R free
0.163 0.161 0.195
Expression system: Escherichia coli

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