4m47

X-ray diffraction
2.37Å resolution

structure of human DNA polymerase complexed with 8-BrG in the template base paired with incoming non-hydrolyzable GTP

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for promutagenicity of 8-halogenated guanine.
J Biol Chem 289 6289-98 (2014)
PMID: 24425881

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-117829 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 12-335; Coverage: 97%)
Gene name: POLB
Sequence domains:
Structure domains:
a synthetic template Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: Homo sapiens
Expression system: Not provided
a synthetic upstream primer Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
a synthetic downstream primer Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 55.175Å b: 79.267Å c: 55.294Å
α: 90° β: 107.8° γ: 90°
R-values:
R R work R free
0.212 0.209 0.272
Expression systems:
  • Escherichia coli
  • Not provided