› 4m9d

X-ray diffraction
1.82Å resolution

The Crystal structure of an adenylosuccinate synthetase from Bacillus anthracis str. Ames Ancestor in complex with AMP.

Released:
DOI: 10.2210/pdb4m9d/pdb
PDB entry 4m9d coloured by chain, front view.
PDB entry 4m9d coloured by chain, side view.
PDB entry 4m9d coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Tan K, Zhou M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 47.98 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81JI9 (Residues: 1-429; Coverage: 100%)
Gene names: BAS5320, BA_5716, GBAA_5716, purA
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand AMP 2 x AMP
Ligand MLI 3 x MLI
Ligand EDO 6 x EDO
Ligand FMT 3 x FMT
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 128.045Å b: 255.947Å c: 61.131Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.182
Expression system: Escherichia coli BL21(DE3)

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