› 4m9z

X-ray diffraction
3.4Å resolution

Crystal structure of CED-4 bound CED-3 fragment

Released:
DOI: 10.2210/pdb4m9z/pdb
PDB entry 4m9z coloured by chain, front view.
PDB entry 4m9z coloured by chain, side view.
PDB entry 4m9z coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Mechanistic insights into CED-4-mediated activation of CED-3.
Huang W, Jiang T, Choi W, Qi S, Pang Y, Hu Q, Xu Y, Gong X, Jeffrey PD, Wang J, Shi Y
OpenAccess logo Genes Dev 27 2039-48 (2013)
PMID: 24065769

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 32-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151739 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell death protein 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 549 amino acids
Theoretical weight: 63.8 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P30429 (Residues: 1-549; Coverage: 100%)
Gene names: C35D10.9, ced-4
Sequence domains:
Structure domains:
CED-3 fragment Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 8 amino acids
Theoretical weight: 975 Da
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ATP 4 x ATP
1 modified residue:
Ligand MSE 76 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P4212
Unit cell:
a: 178.167Å b: 178.167Å c: 200.738Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.27 0.327
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

New insights into apoptosome structure and function.
Dorstyn et al. (2018)
6 more

1 mention without citation

Mechanistic insights into CED-4-mediated activation of CED-3.
Huang et al. (2013)