› 4mag

X-ray diffraction
1.45Å resolution

Crystal structure of the Periplasmic Sialic Acid Binding Protein from Vibrio Cholerea

Released:
DOI: 10.2210/pdb4mag/pdb
PDB entry 4mag coloured by chain, front view.
PDB entry 4mag coloured by chain, side view.
PDB entry 4mag coloured by chain, top view.
Source organism: Vibrio cholerae MJ-1236
Primary publication:
Bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site.
Gangi Setty T, Cho C, Govindappa S, Apicella MA, Ramaswamy S
OpenAccess logo Acta Crystallogr D Biol Crystallogr 70 1801-11 (2014)
PMID: 25004958

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialic Acid Binding Protein Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 34.86 KDa
Source organism: Vibrio cholerae MJ-1236
Expression system: Escherichia coli BL21(DE3)
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

2 bound ligands:
Ligand CO 1 x CO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 74.977Å b: 82.315Å c: 119.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

"Just a spoonful of sugar...": import of sialic acid across bacterial cell membranes.
North et al. (2018)
3 more

6 mentions without citation

Biochemical and structural basis of sialic acid utilization by gut microbes.
Bell et al. (2023)
5 more