› 4mf9

X-ray diffraction
1.95Å resolution

Crystal structure of holo-PhuS, a heme-binding protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4mf9/pdb
PDB entry 4mf9 coloured by chain, front view.
PDB entry 4mf9 coloured by chain, side view.
PDB entry 4mf9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural analysis and identification of PhuS as a heme-degrading enzyme from Pseudomonas aeruginosa.
Lee MJ, Schep D, McLaughlin B, Kaufmann M, Jia Z
J Mol Biol 426 1936-46 (2014)
PMID: 24560694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haemin-degrading HemS/ChuX domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 39.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: O68880 (Residues: 1-354; Coverage: 100%)
Gene name: phuS
Sequence domains: Haemin-degrading HemS.ChuX domain
Structure domains: HemS/ChuS/ChuX like domains

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I4
Unit cell:
a: 187Å b: 187Å c: 42.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Heme Synthesis and Acquisition in Bacterial Pathogens.
Choby et al. (2016)
6 other articles

5 mentions without citation

From Host Heme To Iron: The Expanding Spectrum of Heme Degrading Enzymes Used by Pathogenic Bacteria.
Lyles et al. (2018)
4 more