› 4mfr

X-ray diffraction
2.5Å resolution

Crystal structure of Mycobacterium tuberculosis CarD

Released:
DOI: 10.2210/pdb4mfr/pdb
PDB entry 4mfr coloured by chain, front view.
PDB entry 4mfr coloured by chain, side view.
PDB entry 4mfr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture.
Kaur G, Dutta D, Thakur KG
Proteins 82 879-84 (2014)
PMID: 24115125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase-binding transcription factor CarD Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 18.99 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJG3 (Residues: 1-162; Coverage: 100%)
Gene names: Rv3583c, carD
Sequence domains:
Structure domains: CarD-like, C-terminal domain

Ligands and Environments

3 bound ligands:
Ligand IOD 16 x IOD
Ligand GOL 1 x GOL
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 93.248Å b: 93.248Å c: 87.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.243
Expression system: Escherichia coli

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Citations

1 review citation

Legume genomics and transcriptomics: From classic breeding to modern technologies.
Afzal et al. (2020)
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