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A highly conserved cysteine of neuronal calcium-sensing proteins controls cooperative binding of Ca2+ to recoverin.

Ranaghan MJ, Kumar RP, Chakrabarti KS, Buosi V, Kern D, Oprian DD
J Biol Chem 288 36160-7 (2013)
Related entries: 4m2o, 4m2p, 4m2q

Cited: 15 times
EuropePMC logo PMID: 24189072

Abstract

Recoverin, a 23-kDa Ca(2+)-binding protein of the neuronal calcium sensing (NCS) family, inhibits rhodopsin kinase, a Ser/Thr kinase responsible for termination of photoactivated rhodopsin in rod photoreceptor cells. Recoverin has two functional EF hands and a myristoylated N terminus. The myristoyl chain imparts cooperativity to the Ca(2+)-binding sites through an allosteric mechanism involving a conformational equilibrium between R and T states of the protein. Ca(2+) binds preferentially to the R state; the myristoyl chain binds preferentially to the T state. In the absence of myristoylation, the R state predominates, and consequently, binding of Ca(2+) to the non-myristoylated protein is not cooperative. We show here that a mutation, C39A, of a highly conserved Cys residue among NCS proteins, increases the apparent cooperativity for binding of Ca(2+) to non-myristoylated recoverin. The binding data can be explained by an effect on the T/R equilibrium to favor the T state without affecting the intrinsic binding constants for the two Ca(2+) sites.

Articles - 4mlw mentioned but not cited (4)

  1. Diversification of fungal specific class a glutathione transferases in saprotrophic fungi. Mathieu Y, Prosper P, Favier F, Harvengt L, Didierjean C, Jacquot JP, Morel-Rouhier M, Gelhaye E. PLoS One 8 e80298 (2013)
  2. A highly conserved cysteine of neuronal calcium-sensing proteins controls cooperative binding of Ca2+ to recoverin. Ranaghan MJ, Kumar RP, Chakrabarti KS, Buosi V, Kern D, Oprian DD. J Biol Chem 288 36160-36167 (2013)
  3. Crystal Structure of Recoverin with Calcium Ions Bound to Both Functional EF Hands. Kumar RP, Ranaghan MJ, Ganjei AY, Oprian DD. Biochemistry 54 7222-7228 (2015)
  4. Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes. Baksheeva VE, Tsvetkov PO, Zalevsky AO, Vladimirov VI, Gorokhovets NV, Zinchenko DV, Permyakov SE, Devred F, Zernii EY. Biomolecules 12 956 (2022)


Reviews citing this publication (3)

  1. Biological chemistry and functionality of protein sulfenic acids and related thiol modifications. Devarie-Baez NO, Silva Lopez EI, Furdui CM. Free Radic Res 50 172-194 (2016)
  2. The Binding Properties and Physiological Functions of Recoverin. Zang J, Neuhauss SCF. Front Mol Neurosci 11 473 (2018)
  3. Caldendrin and Calneurons-EF-Hand CaM-Like Calcium Sensors With Unique Features and Specialized Neuronal Functions. Mundhenk J, Fusi C, Kreutz MR. Front Mol Neurosci 12 16 (2019)

Articles citing this publication (8)

  1. Conformational Selection in a Protein-Protein Interaction Revealed by Dynamic Pathway Analysis. Chakrabarti KS, Agafonov RV, Pontiggia F, Otten R, Higgins MK, Schertler GFX, Oprian DD, Kern D. Cell Rep 14 32-42 (2016)
  2. Light-induced disulfide dimerization of recoverin under ex vivo and in vivo conditions. Zernii EY, Nazipova AA, Gancharova OS, Kazakov AS, Serebryakova MV, Zinchenko DV, Tikhomirova NK, Senin II, Philippov PP, Permyakov EA, Permyakov SE. Free Radic Biol Med 83 283-295 (2015)
  3. Light-Induced Thiol Oxidation of Recoverin Affects Rhodopsin Desensitization. Zernii EY, Nazipova AA, Nemashkalova EL, Kazakov AS, Gancharova OS, Serebryakova MV, Tikhomirova NK, Baksheeva VE, Vladimirov VI, Zinchenko DV, Philippov PP, Senin II, Permyakov SE. Front Mol Neurosci 11 474 (2018)
  4. Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein. Yang ST, Lim SI, Kiessling V, Kwon I, Tamm LK. Sci Rep 6 32866 (2016)
  5. Oxidative stress sensing and response in neural stem cell fate. Hwang I, Tang D, Paik J. Free Radic Biol Med 169 74-83 (2021)
  6. Disulfide Dimerization of Neuronal Calcium Sensor-1: Implications for Zinc and Redox Signaling. Baksheeva VE, Baldin AV, Zalevsky AO, Nazipova AA, Kazakov AS, Vladimirov VI, Gorokhovets NV, Devred F, Philippov PP, Bazhin AV, Golovin AV, Zamyatnin AA, Zinchenko DV, Tsvetkov PO, Permyakov SE, Zernii EY. Int J Mol Sci 22 12602 (2021)
  7. Bringing the Ca2+ sensitivity of myristoylated recoverin into the physiological range. Marino V, Riva M, Zamboni D, Koch KW, Dell'Orco D. Open Biol 11 200346 (2021)
  8. Redox Regulation of Signaling Complex between Caveolin-1 and Neuronal Calcium Sensor Recoverin. Vladimirov VI, Shchannikova MP, Baldin AV, Kazakov AS, Shevelyova MP, Nazipova AA, Baksheeva VE, Nemashkalova EL, Frolova AS, Tikhomirova NK, Philippov PP, Zamyatnin AA, Permyakov SE, Zinchenko DV, Zernii EY. Biomolecules 12 1698 (2022)


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