› 4mpq

X-ray diffraction
1.75Å resolution

Crystal structure of1-pyrroline-4-hydroxy-2-carboxylate deaminase from Brucella melitensis ATCC 23457

Released:
DOI: 10.2210/pdb4mpq/pdb
PDB entry 4mpq coloured by chain, front view.
PDB entry 4mpq coloured by chain, side view.
PDB entry 4mpq coloured by chain, top view.
Source organism: Brucella melitensis ATCC 23457
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
1-pyrroline-4-hydroxy-2-carboxylate + H(2)O = 2,5-dioxopentanoate + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrodipicolinate synthetase Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 35.66 KDa
Source organism: Brucella melitensis ATCC 23457
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0RKH4 (Residues: 2-320; Coverage: 100%)
Gene name: BMEA_B0240
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6322
Unit cell:
a: 95.06Å b: 95.06Å c: 125.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.159 0.185
Expression system: Escherichia coli BL21(DE3)

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