› 4ms3

X-ray diffraction
2.5Å resolution

Crystal structure of the extracellular domain of human GABA(B) receptor bound to the endogenous agonist GABA

Released:
DOI: 10.2210/pdb4ms3/pdb
PDB entry 4ms3 coloured by chain, front view.
PDB entry 4ms3 coloured by chain, side view.
PDB entry 4ms3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism of ligand activation in human GABA(B) receptor.
Geng Y, Bush M, Mosyak L, Wang F, Fan QR
OpenAccess logo Nature 504 254-9 (2013)
PMID: 24305054

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131164 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gamma-aminobutyric acid type B receptor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UBS5 (Residues: 165-576; Coverage: 44%)
  • Best match: Q9UBS5-5 (Residues: 165-569)
Gene names: GABBR1, GPRC3A
Sequence domains: Receptor family ligand binding region
Structure domains: Rossmann fold
Gamma-aminobutyric acid type B receptor subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 433 amino acids
Theoretical weight: 49.13 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75899 (Residues: 42-466; Coverage: 47%)
Gene names: GABBR2, GPR51, GPRC3B
Sequence domains: Receptor family ligand binding region
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand ABU 1 x ABU
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 115.48Å b: 141.69Å c: 59.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.232
Expression system: Spodoptera frugiperda

Quick links

Citations

29 review citations

Mechanisms of signalling and biased agonism in G protein-coupled receptors.
Wootten et al. (2018)
28 more

16 mentions without citation

Structural biology of GABAB receptor.
Frangaj et al. (2018)
15 more