› 4msw

X-ray diffraction
2.06Å resolution

Y78 ester mutant of KcsA in high K+

Released:
DOI: 10.2210/pdb4msw/pdb
PDB entry 4msw coloured by chain, front view.
PDB entry 4msw coloured by chain, side view.
PDB entry 4msw coloured by chain, top view.
Source organisms:
Primary publication:
Using protein backbone mutagenesis to dissect the link between ion occupancy and C-type inactivation in K+ channels.
Matulef K, Komarov AG, Costantino CA, Valiyaveetil FI
Proc Natl Acad Sci U S A 110 17886-91 (2013)
PMID: 24128761

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141094 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 10.99 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 22-124; Coverage: 64%)
Gene names: kcsA, skc1
Sequence domains:
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand DGA 1 x DGA
Ligand K 6 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: I4
Unit cell:
a: 154.53Å b: 154.53Å c: 75.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.229
Expression systems:
  • Komagataella pastoris
  • Escherichia coli

Quick links

Citations

6 review citations

Chemical synthesis and biophysical applications of membrane proteins.
Zuo et al. (2015)
5 more

5 mentions without citation

Improving the accuracy of the structure prediction of the third hypervariable loop of the heavy chains of antibodies.
Messih et al. (2014)
4 more