› 4mxv

X-ray diffraction
3.2Å resolution

Structure of Lymphotoxin alpha bound to anti-LTa Fab

Released:
DOI: 10.2210/pdb4mxv/pdb
PDB entry 4mxv coloured by chain, front view.
PDB entry 4mxv coloured by chain, side view.
PDB entry 4mxv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dimerization of LTβR by LTα1β2 is necessary and sufficient for signal transduction.
Sudhamsu J, Yin J, Chiang EY, Starovasnik MA, Grogan JL, Hymowitz SG
Proc Natl Acad Sci U S A 110 19896-901 (2013)
PMID: 24248355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208417 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lymphotoxin-alpha Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 157 amino acids
Theoretical weight: 17.31 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01374 (Residues: 62-205; Coverage: 84%)
Gene names: LTA, TNFB, TNFSF1
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
anti-Lymphotoxin alpha antibody light chain Chains: E, L, X
Molecule details ›
Chains: E, L, X
Length: 211 amino acids
Theoretical weight: 23.07 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
anti-Lymphotoxin alpha antibody heavy chain Chains: F, H, Y
Molecule details ›
Chains: F, H, Y
Length: 213 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 209.847Å b: 209.847Å c: 130.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.177 0.235
Expression systems:
  • Trichoplusia ni
  • Cricetulus griseus

Quick links

Citations

7 review citations

Structural principles of tumor necrosis factor superfamily signaling.
Vanamee et al. (2018)
6 more

2 mentions without citation

Evaluating the Use of Antibody Variable Region (Fv) Charge as a Risk Assessment Tool for Predicting Typical Cynomolgus Monkey Pharmacokinetics.
Bumbaca Yadav et al. (2015)
1 more