› 4mz1

X-ray diffraction
2.4Å resolution

Crystal Structure of the Inosine 5'-monophosphate Dehydrogenase, with a Internal Deletion of CBS Domain from Campylobacter jejuni complexed with inhibitor compound P12

Released:
DOI: 10.2210/pdb4mz1/pdb
PDB entry 4mz1 coloured by chain, front view.
PDB entry 4mz1 coloured by chain, side view.
PDB entry 4mz1 coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Kim Y, Makowska-Grzyska M, Gu M, Anderson WF, Joachimiak A, CSGID, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-555497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 385 amino acids
Theoretical weight: 41.05 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q0P9J4 (Residues: 1-91, 93-485; Coverage: 79%)
Gene names: Cj1058c, guaB
Sequence domains: IMP dehydrogenase / GMP reductase domain
Structure domains: Aldolase class I

Ligands and Environments

6 bound ligands:
Ligand IMP 3 x IMP
Ligand 2F1 3 x 2F1
Ligand K 3 x K
Ligand SO4 4 x SO4
Ligand PO4 6 x PO4
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I422
Unit cell:
a: 118.062Å b: 118.062Å c: 435.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.212
Expression system: Escherichia coli BL21

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