› 4n0h

X-ray diffraction
1.95Å resolution

Crystal structure of S. cerevisiae mitochondrial GatFAB

Released:
DOI: 10.2210/pdb4n0h/pdb
PDB entry 4n0h coloured by chain, front view.
PDB entry 4n0h coloured by chain, side view.
PDB entry 4n0h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of Saccharomyces cerevisiae mitochondrial GatFAB reveals a novel subunit assembly in tRNA-dependent amidotransferases.
Araiso Y, Huot JL, Sekiguchi T, Frechin M, Fischer F, Enkler L, Senger B, Ishitani R, Becker HD, Nureki O
OpenAccess logo Nucleic Acids Res 42 6052-63 (2014)
PMID: 24692665

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152655 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 50.98 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03557 (Residues: 1-464; Coverage: 100%)
Gene names: GEP6, HER2, LRC6, YMR293C
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 325 amino acids
Theoretical weight: 36.7 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33893 (Residues: 16-329; Coverage: 58%)
Gene names: PET112, YBL0724, YBL080C
Sequence domains: GatB/GatE catalytic domain
Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial Chain: F
Molecule details ›
Chain: F
Length: 160 amino acids
Theoretical weight: 18.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53260 (Residues: 24-183; Coverage: 87%)
Gene names: GTF1, YGR102C
Sequence domains: Glutamyl-tRNA(Gln) amidotransferase subunit F

Ligands and Environments

1 bound ligand:
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 61.949Å b: 86.297Å c: 191.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Aminoacyl-tRNA synthetases.
Rubio Gomez et al. (2020)
2 more

3 mentions without citation

Architecture of the mycobacterial type VII secretion system.
Famelis et al. (2019)
2 more