› 4n0w

X-ray diffraction
1.65Å resolution

X-ray crystal structure of a serine hydroxymethyltransferase from Burkholderia cenocepacia with covalently attached pyridoxal phosphate

Released:
DOI: 10.2210/pdb4n0w/pdb
PDB entry 4n0w coloured by chain, front view.
PDB entry 4n0w coloured by chain, side view.
PDB entry 4n0w coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 424 amino acids
Theoretical weight: 46.07 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4ECY9 (Residues: 1-415; Coverage: 100%)
Gene names: BCAL3197, glyA
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 65.58Å b: 178.65Å c: 75.66Å
α: 90° β: 114.7° γ: 90°
R-values:
R R work R free
0.157 0.156 0.175
Expression system: Escherichia coli

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