› 4n8h

X-ray diffraction
2.4Å resolution

E61V mutant, RipA structure

Released:
DOI: 10.2210/pdb4n8h/pdb
PDB entry 4n8h coloured by chain, front view.
PDB entry 4n8h coloured by chain, side view.
PDB entry 4n8h coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Structural snapshots along the reaction pathway of Yersinia pestis RipA, a putative butyryl-CoA transferase.
Torres R, Lan B, Latif Y, Chim N, Goulding CW
Acta Crystallogr D Biol Crystallogr 70 1074-85 (2014)
PMID: 24699651

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxybutyrate coenzyme A transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 476 amino acids
Theoretical weight: 52.25 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZC36 (Residues: 1-440; Coverage: 100%)
Gene name: YPO1926
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 118.907Å b: 109.003Å c: 85.014Å
α: 90° β: 120.07° γ: 90°
R-values:
R R work R free
0.154 0.151 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

The activating oxydianion binding domain for enzyme-catalyzed proton transfer, hydride transfer, and decarboxylation: specificity and enzyme architecture.
Reyes et al. (2015)
2 more

1 mention without citation

Structural snapshots along the reaction pathway of Yersinia pestis RipA, a putative butyryl-CoA transferase.
Torres et al. (2014)