› 4n9f

X-ray diffraction
3.3Å resolution

Crystal structure of the Vif-CBFbeta-CUL5-ElOB-ElOC pentameric complex

Released:
DOI: 10.2210/pdb4n9f/pdb
Model geometry
Fit model/data
PDB entry 4n9f coloured by chain, front view.
PDB entry 4n9f coloured by chain, side view.
PDB entry 4n9f coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for hijacking CBF-b and CUL5 E3 ligase complex by HIV-1 Vif
Guo YY, Dong LY, Qiu XL, Wang YS, Zhang BL, Liu HN, Yu Y, Zang Y, Yang MJ, Huang ZW
Nature 505 229-233 (2014)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-146244 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cullin-5 Chains: 3, 9, C, I, O, U, V, f, l, r, w, x
Molecule details ›
Chains: 3, 9, C, I, O, U, V, f, l, r, w, x
Length: 311 amino acids
Theoretical weight: 36.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93034 (Residues: 12-321; Coverage: 40%)
Gene names: CUL5, VACM1
Sequence domains: Cullin family
Structure domains: Cullin Repeats
Elongin-C Chains: 5, B, E, K, Q, T, Y, Z, h, n, t, z
Molecule details ›
Chains: 5, B, E, K, Q, T, Y, Z, h, n, t, z
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
Elongin-B Chains: 4, D, H, J, P, W, X, e, g, m, s, y
Core-binding factor subunit beta Chains: 0, 6, F, L, N, R, a, c, i, k, o, u
Virion infectivity factor Chains: 1, 2, 7, G, M, S, b, d, j, p, q, v

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 103.925Å b: 204.002Å c: 247.866Å
α: 65.51° β: 90.28° γ: 90.5°
R-values:
R R work R free
0.277 0.262 0.324
Expression system: Escherichia coli

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