4n9i

X-ray diffraction
2.19Å resolution

Crystal Structure of Transcription regulation protein CRP complexed with cGMP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-111203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catabolite activator protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 210 amino acids
Theoretical weight: 23.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: C3SQJ7 (Residues: 1-210; Coverage: 100%)
Gene names: A2J79_001505, A5U30_003905, ABE91_008870, ACU57_16420, AWP47_12490, B6R15_000427, B6R31_002254, BANRA_01989, BANRA_04354, BCB93_002475, BG944_001159, BGM66_002213, BGZ_04165, BK383_21300, BKL28_001894, BMT50_12075, BMT91_03425, BRV02_001753, BTB68_000020, BTQ06_10840, BXT93_05030, BvCmsKKP061_02735, C0P57_000760, C1Q91_001758, C2M16_11070, C2R31_002864, C3F40_19505, C6669_11215, C719_002225, C7B02_12645, C9160_13365, C9194_03755, C9Z68_19280, CCS08_20135, CF22_001817, CG704_07735, CIG67_21165, CR538_02195, CTR35_001840, CV83915_02753, CWS33_10910, D3G36_06735, D4N09_12870, D9D43_04095, D9E49_05440, D9J61_04535, DD762_11900, DIV22_04510, DL968_00110, DNQ45_16825, DNX30_18290, DS732_24565, DTL43_13855, DU321_12215, E2863_04750, E5H86_09300, E6D34_04090, ECs4208, EIA08_11980, EIZ93_01465, EN85_000612, EPS76_12615, EPS97_04750, EWK56_16140, EXX87_01840, ExPECSC038_01191, F7F11_11960, F7N46_14555, F9413_17550, F9461_02940, F9B07_12715, FGAF848_25590, FIJ20_20760, FJQ40_03300, FKO60_13850, FOI11_018975, FOI11_04205, FPI65_20600, FPS11_03485, FVB16_17495, FZU14_04085, G3V95_05875, G3W53_10710, G4A38_16795, G4A47_18385, G5603_11130, GAI89_06415, GAJ12_06845, GGB84_001611, GKF66_04625, GNW61_19905, GNZ05_15775, GOP25_12350, GP711_17510, GP944_08120, GP954_11630, GP965_06375, GP975_05705, GQE64_08005, GQE86_19765, GQM04_08950, GQM13_20765, GQM21_13125, GQN24_10635, GRW24_20180, GTP92_12845, GUC01_14505, HEP30_005360, HEP34_002431, HHH44_000564, HI055_002212, HIE29_002022, HJQ60_002820, HKA49_001089, HLX92_09860, HLZ39_07535, HLZ50_06700, HV109_01905, HV209_14500, HVV39_09550, HVW04_17320, HVW43_18455, HVY77_01890, I6H02_12205, IFC14_001574, IH772_08930, J0541_002108, J5U05_000715, J8F57_000447, JNA68_03125, JNP96_25590, NCTC10082_02782, NCTC10279_02134, NCTC10418_00642, NCTC10429_01028, NCTC10767_01464, NCTC10865_00613, NCTC10974_00491, NCTC11126_02705, NCTC11181_02696, NCTC11341_01936, NCTC7927_00493, NCTC7928_02562, NCTC8179_05897, NCTC8333_00446, NCTC8500_00264, NCTC8603_03318, NCTC8621_00464, NCTC8622_00559, NCTC8960_03028, NCTC9001_04188, NCTC9044_01404, NCTC9045_00499, NCTC9073_00445, NCTC9075_00660, NCTC9702_00492, NCTC9706_02630, NOI85_11600, NY836_11640, OFN31_07325, OGM49_22615, P6223_000183, PWL68_002688, Q2V20_09420, Q2V64_17715, QDW62_01910, QO046_13970, R8G00_13540, R8O40_002176, RZR61_13175, SAMEA3472044_01106, SAMEA3752557_02008, V9Z47_02200, WR15_02730, crp
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 42.568Å b: 56.171Å c: 186.718Å
α: 90° β: 90.18° γ: 90°
R-values:
R R work R free
0.172 0.17 0.208
Expression system: Escherichia coli