› 4nco

X-ray diffraction
4.7Å resolution

Crystal Structure of the BG505 SOSIP gp140 HIV-1 Env trimer in Complex with the Broadly Neutralizing Fab PGT122

Released:
DOI: 10.2210/pdb4nco/pdb
PDB entry 4nco coloured by chain, front view.
PDB entry 4nco coloured by chain, side view.
PDB entry 4nco coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a soluble cleaved HIV-1 envelope trimer.
Julien JP, Cupo A, Sok D, Stanfield RL, Lyumkis D, Deller MC, Klasse PJ, Burton DR, Sanders RW, Moore JP, Ward AB, Wilson IA
Science 342 1477-83 (2013)
PMID: 24179159

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211665 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Envelope glycoprotein gp160 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 475 amino acids
Theoretical weight: 53.12 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-504; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
BG505 SOSIP gp41 Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 78 amino acids
Theoretical weight: 6.66 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
PGT122 light chain Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 211 amino acids
Theoretical weight: 22.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PGT122 heavy chain Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 235 amino acids
Theoretical weight: 25.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 30 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 152.2Å b: 260.72Å c: 283.18Å
α: 90° β: 99.56° γ: 90°
R-values:
R R work R free
0.376 0.375 0.389
Expression system: Homo sapiens

Quick links

Citations

119 review citations

Biological roles of glycans.
Varki A. (2017)
118 more

103 mentions without citation

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
102 more