› 4nk1

X-ray diffraction
2.21Å resolution

Crystal structure of phosphate-bound Hell's gate globin IV

Released:
DOI: 10.2210/pdb4nk1/pdb
PDB entry 4nk1 coloured by chain, front view.
PDB entry 4nk1 coloured by chain, side view.
PDB entry 4nk1 coloured by chain, top view.
Source organism: Methylacidiphilum infernorum V4
Primary publication:
Crystal structure of truncated haemoglobin from an extremely thermophilic and acidophilic bacterium.
Jamil F, Teh AH, Schadich E, Saito JA, Najimudin N, Alam M
J Biochem 156 97-106 (2014)
PMID: 24733432

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemoglobin-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 22.7 KDa
Source organism: Methylacidiphilum infernorum V4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3DVC3 (Residues: 1-193; Coverage: 100%)
Gene name: Minf_1222
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 68.258Å b: 84.485Å c: 92.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Open and Lys-His Hexacoordinated Closed Structures of a Globin with Swapped Proximal and Distal Sites.
Teh et al. (2015)