› 4npd

X-ray diffraction
0.9Å resolution

High-resolution structure of C domain of staphylococcal protein A at cryogenic temperature

Released:
DOI: 10.2210/pdb4npd/pdb
PDB entry 4npd coloured by chain, front view.
PDB entry 4npd coloured by chain, side view.
PDB entry 4npd coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Multiscale conformational heterogeneity in staphylococcal protein a: possible determinant of functional plasticity.
Deis LN, Pemble CW, Qi Y, Hagarman A, Richardson DC, Richardson JS, Oas TG
Structure 22 1467-77 (2014)
PMID: 25295398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein A Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.64 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38507 (Residues: 270-327; Coverage: 12%)
Gene name: spa
Sequence domains: B domain
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SCN 1 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 27.25Å b: 38.41Å c: 28.61Å
α: 90° β: 117.44° γ: 90°
R-values:
R R work R free
0.113 0.112 0.13
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Functional roles of transiently and intrinsically disordered regions within proteins.
Uversky VN. (2015)
2 more

5 mentions without citation

Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies).
Wu et al. (2021)
4 more