› 4nt2

X-ray diffraction
2.4Å resolution

Crystal structure of Arabidopsis ACD11 (accelerated-cell-death 11) complexed with lyso-sphingomyelin (d18:1) at 2.4 Angstrom resolution

Released:
DOI: 10.2210/pdb4nt2/pdb
PDB entry 4nt2 coloured by chain, front view.
PDB entry 4nt2 coloured by chain, side view.
PDB entry 4nt2 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels.
Simanshu DK, Zhai X, Munch D, Hofius D, Markham JE, Bielawski J, Bielawska A, Malinina L, Molotkovsky JG, Mundy JW, Patel DJ, Brown RE
Cell Rep 6 388-99 (2014)
PMID: 24412362

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Accelerated cell death 11 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 22.8 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O64587 (Residues: 1-206; Coverage: 100%)
Gene names: ACD11, At2g34690, T29F13.10
Sequence domains: Glycolipid transfer protein (GLTP)
Structure domains: Glycolipid transfer protein

Ligands and Environments

3 bound ligands:
Ligand SPU 2 x SPU
Ligand SO4 5 x SO4
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P6322
Unit cell:
a: 108.827Å b: 108.827Å c: 105.007Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.191 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Lipid signalling in plant responses to abiotic stress.
Hou et al. (2016)
14 more

3 mentions without citation

Emerging roles for human glycolipid transfer protein superfamily members in the regulation of autophagy, inflammation, and cell death.
Mishra et al. (2020)
2 more