› 4nu2

X-ray diffraction
2.1Å resolution

Crystal structure of an ice-binding protein (FfIBP) from the Antarctic bacterium, Flavobacterium frigoris PS1

Released:
DOI: 10.2210/pdb4nu2/pdb
PDB entry 4nu2 coloured by chain, front view.
PDB entry 4nu2 coloured by chain, side view.
PDB entry 4nu2 coloured by chain, top view.
Source organism: Flavobacterium frigoris PS1
Primary publication:
Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein from the Antarctic bacterium Flavobacterium frigoris PS1.
Do H, Kim SJ, Kim HJ, Lee JH
Acta Crystallogr D Biol Crystallogr 70 1061-73 (2014)
PMID: 24699650

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ice-binding protein Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 25.46 KDa
Source organism: Flavobacterium frigoris PS1
Expression system: Escherichia coli
UniProt:
  • Canonical: H7FWB6 (Residues: 29-276; Coverage: 98%)
Gene name: HJ01_03463
Sequence domains: Ice-binding-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P4122
Unit cell:
a: 69.171Å b: 69.171Å c: 178.764Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.208 0.239
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.
Kim et al. (2017)
6 more

5 mentions without citation

Structure and Properties of a Natural Competence-Associated Pilin Suggest a Unique Pilus Tip-Associated DNA Receptor.
Salleh et al. (2019)
4 more