› 4nu5

X-ray diffraction
2.35Å resolution

Crystal Structure of PTDH R301A

Released:
DOI: 10.2210/pdb4nu5/pdb
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PDB entry 4nu5 coloured by chain, front view.
PDB entry 4nu5 coloured by chain, side view.
PDB entry 4nu5 coloured by chain, top view.
Source organism: Pseudomonas stutzeri
Primary publication:
Chemical rescue and inhibition studies to determine the role of Arg301 in phosphite dehydrogenase.
Hung JE, Fogle EJ, Garg N, Chekan JR, Nair SK, van der Donk WA
OpenAccess logo PLoS One 9 e87134 (2014)
PMID: 24498026

Function and Biology Details

Reaction catalysed: 
Phosphonate + NAD(+) + H(2)O = phosphate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphonate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 35.77 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli
UniProt:
  • Canonical: O69054 (Residues: 1-329; Coverage: 98%)
Gene name: ptxD
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 116.486Å b: 80.456Å c: 81.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.23
Expression system: Escherichia coli

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Citations

8 citation in other articles

Guanidine hydrochloride reactivates an ancient septin hetero-oligomer assembly pathway in budding yeast.
Johnson et al. (2020)
7 more

1 mention without citation

Chemical rescue and inhibition studies to determine the role of Arg301 in phosphite dehydrogenase.
Hung et al. (2014)

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