› 4nzh

X-ray diffraction
2Å resolution

A. fumigatus flavin-dependent ornithine monooxygenase R279A mutant

Released:
DOI: 10.2210/pdb4nzh/pdb
PDB entry 4nzh coloured by chain, front view.
PDB entry 4nzh coloured by chain, side view.
PDB entry 4nzh coloured by chain, top view.
Source organism: Aspergillus fumigatus
Entry authors: Robinson R, Franceschini S, Sobrado P

Function and Biology Details

Reaction catalysed: 
L-ornithine + NAD(P)H + O(2) = N(5)-hydroxy-L-ornithine + NAD(P)(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123229 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-ornithine N(5)-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 56.87 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E9QYP0 (Residues: 1-501; Coverage: 100%)
Gene names: Afu2g07680, sidA
Sequence domains: L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand ORN 1 x ORN
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I222
Unit cell:
a: 77.905Å b: 84.083Å c: 144.902Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.243
Expression system: Escherichia coli BL21(DE3)

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