› 4o3b

X-ray diffraction
1.91Å resolution

Crystal structure of an open/closed glua2 ligand-binding domain dimer at 1.91 A resolution

Released:
DOI: 10.2210/pdb4o3b/pdb
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PDB entry 4o3b coloured by chain, front view.
PDB entry 4o3b coloured by chain, side view.
PDB entry 4o3b coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
L-Asp is a useful tool in the purification of the ionotropic glutamate receptor A2 ligand-binding domain.
Krintel C, Frydenvang K, Ceravalls de Rabassa A, Kaern AM, Gajhede M, Pickering DS, Kastrup JS
FEBS J 281 2422-30 (2014)
PMID: 24673938

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 29.22 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

7 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 6 x GOL
Ligand CL 1 x CL
Ligand PEG 1 x PEG
Ligand ACT 1 x ACT
Ligand GLU 1 x GLU
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MAX II BEAMLINE I911-5
Spacegroup: P212121
Unit cell:
a: 62.72Å b: 93.72Å c: 97.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.178
Expression system: Escherichia coli

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Citations

4 citation in other articles

Functional Validation of Heteromeric Kainate Receptor Models.
Paramo et al. (2017)
3 more

2 mentions without citation

Prediction of Ordered Water Molecules in Protein Binding Sites from Molecular Dynamics Simulations: The Impact of Ligand Binding on Hydration Networks.
Rudling et al. (2018)
1 more

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