› 4o3t

X-ray diffraction
2.99Å resolution

Zymogen HGF-beta/MET with Zymogen Activator Peptide ZAP.14

Released:
DOI: 10.2210/pdb4o3t/pdb
PDB entry 4o3t coloured by chain, front view.
PDB entry 4o3t coloured by chain, side view.
PDB entry 4o3t coloured by chain, top view.
Source organisms:
Primary publication:
An allosteric switch for pro-HGF/Met signaling using zymogen activator peptides.
Landgraf KE, Steffek M, Quan C, Tom J, Yu C, Santell L, Maun HR, Eigenbrot C, Lazarus RA
Nat Chem Biol 10 567-73 (2014)
PMID: 24859116

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-140189 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Hepatocyte growth factor beta chain Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 26.8 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14210 (Residues: 495-728; Coverage: 34%)
Gene names: HGF, HPTA
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hepatocyte growth factor receptor Chain: B
Molecule details ›
Chain: B
Length: 551 amino acids
Theoretical weight: 62.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08581 (Residues: 25-567; Coverage: 40%)
Gene name: MET
Sequence domains:
Structure domains:
ZAP.14 Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.32 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 59.789Å b: 121.467Å c: 137.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.276
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

1 review citation

The cytokines of pulmonary fibrosis: Much learned, much more to learn.
Luzina et al. (2015)
11 other articles

2 mentions without citation

State of the structure address on MET receptor activation by HGF.
Linossi et al. (2021)
1 more