› 4o69

X-ray diffraction
2.25Å resolution

Human cyclic GMP-AMP synthase (cGAS) in complex with sulfate ion

Released:
DOI: 10.2210/pdb4o69/pdb
PDB entry 4o69 coloured by chain, front view.
PDB entry 4o69 coloured by chain, side view.
PDB entry 4o69 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cytosolic DNA sensor cGAS forms an oligomeric complex with DNA and undergoes switch-like conformational changes in the activation loop.
Zhang X, Wu J, Du F, Xu H, Sun L, Chen Z, Brautigam CA, Zhang X, Chen ZJ
Cell Rep 6 421-30 (2014)
PMID: 24462292

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 42.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N884 (Residues: 161-522; Coverage: 69%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 101.027Å b: 101.027Å c: 241.331Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.224
Expression system: Escherichia coli

Quick links

Citations

124 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
123 more

6 mentions without citation

The cytosolic DNA sensor cGAS forms an oligomeric complex with DNA and undergoes switch-like conformational changes in the activation loop.
Zhang et al. (2014)
5 more