4o6y › Citations

Structure and mechanism of a eukaryotic transmembrane ascorbate-dependent oxidoreductase.

Lu P, Ma D, Yan C, Gong X, Du M, Shi Y
Proc Natl Acad Sci U S A 111 1813-8 (2014)
Related entries: 4o79, 4o7g

Cited: 36 times
EuropePMC logo PMID: 24449903

Abstract

Vitamin C, also known as ascorbate, is required in numerous essential metabolic reactions in eukaryotes. The eukaryotic ascorbate-dependent oxidoreductase cytochrome b561 (Cyt b561), a family of highly conserved transmembrane enzymes, plays an important role in ascorbate recycling and iron absorption. Although Cyt b561 was identified four decades ago, its atomic structure and functional mechanism remain largely unknown. Here, we report the high-resolution crystal structures of cytochrome b561 from Arabidopsis thaliana in both substrate-free and substrate-bound states. Cyt b561 forms a homodimer, with each protomer consisting of six transmembrane helices and two heme groups. The negatively charged substrate ascorbate, or monodehydroascorbate, is enclosed in a positively charged pocket on either side of the membrane. Two highly conserved amino acids, Lys(81) and His(106), play an essential role in substrate recognition and catalysis. Our structural and biochemical analyses allow the proposition of a general electron transfer mechanism for members of the Cyt b561 family.

Reviews - 4o6y mentioned but not cited (2)

  1. The recombinant expression systems for structure determination of eukaryotic membrane proteins. He Y, Wang K, Yan N. Protein Cell 5 658-672 (2014)
  2. Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects. Bill RM, von der Haar T. Curr Opin Struct Biol 32 147-155 (2015)

Articles - 4o6y mentioned but not cited (5)

  1. Large-scale determination of previously unsolved protein structures using evolutionary information. Ovchinnikov S, Kinch L, Park H, Liao Y, Pei J, Kim DE, Kamisetty H, Grishin NV, Baker D. Elife 4 e09248 (2015)
  2. Structure and mechanism of a eukaryotic transmembrane ascorbate-dependent oxidoreductase. Lu P, Ma D, Yan C, Gong X, Du M, Shi Y. Proc Natl Acad Sci U S A 111 1813-1818 (2014)
  3. Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate. Ganasen M, Togashi H, Takeda H, Asakura H, Tosha T, Yamashita K, Hirata K, Nariai Y, Urano T, Yuan X, Hamza I, Mauk AG, Shiro Y, Sugimoto H, Sawai H. Commun Biol 1 120 (2018)
  4. Improving prediction of helix-helix packing in membrane proteins using predicted contact numbers as restraints. Li B, Mendenhall J, Nguyen ED, Weiner BE, Fischer AW, Meiler J. Proteins 85 1212-1221 (2017)
  5. Ferric reduction by a CYBDOM protein counteracts increased iron availability in root meristems induced by phosphorus deficiency. Maniero RA, Picco C, Hartmann A, Engelberger F, Gradogna A, Scholz-Starke J, Melzer M, Künze G, Carpaneto A, von Wirén N, Giehl RFH. Nat Commun 15 422 (2024)


Reviews citing this publication (4)

  1. Duodenal cytochrome b (DCYTB) in iron metabolism: an update on function and regulation. Lane DJ, Bae DH, Merlot AM, Sahni S, Richardson DR, Richardson DR. Nutrients 7 2274-2296 (2015)
  2. Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics. D'Aniello C, Cermola F, Patriarca EJ, Minchiotti G. Stem Cells Int 2017 8936156 (2017)
  3. Potentiation of hydrogen peroxide toxicity: From catalase inhibition to stable DNA-iron complexes. Mahaseth T, Kuzminov A. Mutat Res Rev Mutat Res 773 274-281 (2017)
  4. New Perspectives on Iron Uptake in Eukaryotes. Sherman HG, Jovanovic C, Stolnik S, Baronian K, Downard AJ, Rawson FJ. Front Mol Biosci 5 97 (2018)

Articles citing this publication (25)

  1. Scavenging of superoxide by a membrane-bound superoxide oxidase. Lundgren CAK, Sjöstrand D, Biner O, Bennett M, Rudling A, Johansson AL, Brzezinski P, Carlsson J, von Ballmoos C, Högbom M. Nat Chem Biol 14 788-793 (2018)
  2. Expansion of the Major Facilitator Superfamily (MFS) to include novel transporters as well as transmembrane-acting enzymes. Wang SC, Davejan P, Hendargo KJ, Javadi-Razaz I, Chou A, Yee DC, Ghazi F, Lam KJK, Conn AM, Madrigal A, Medrano-Soto A, Saier MH. Biochim Biophys Acta Biomembr 1862 183277 (2020)
  3. Six-Transmembrane Epithelial Antigen of Prostate 1 (STEAP1) Has a Single b Heme and Is Capable of Reducing Metal Ion Complexes and Oxygen. Kim K, Mitra S, Wu G, Berka V, Song J, Yu Y, Poget S, Wang DN, Tsai AL, Zhou M. Biochemistry 55 6673-6684 (2016)
  4. Characterization of a single b-type heme, FAD, and metal binding sites in the transmembrane domain of six-transmembrane epithelial antigen of the prostate (STEAP) family proteins. Kleven MD, Dlakić M, Lawrence CM. J Biol Chem 290 22558-22569 (2015)
  5. Structures of mouse DUOX1-DUOXA1 provide mechanistic insights into enzyme activation and regulation. Sun J. Nat Struct Mol Biol 27 1086-1093 (2020)
  6. Evidences for Cooperative Resonance-Assisted Hydrogen Bonds in Protein Secondary Structure Analogs. Zhou Y, Deng G, Zheng YZ, Xu J, Ashraf H, Yu ZW. Sci Rep 6 36932 (2016)
  7. Mutations in CYB561 Causing a Novel Orthostatic Hypotension Syndrome. van den Berg MP, Almomani R, Biaggioni I, van Faassen M, van der Harst P, Silljé HHW, Mateo Leach I, Hemmelder MH, Navis G, Luijckx GJ, de Brouwer APM, Venselaar H, Verbeek MM, van der Zwaag PA, Jongbloed JDH, van Tintelen JP, Wevers RA, Kema IP. Circ Res 122 846-854 (2018)
  8. Visualizing the protons in a metalloenzyme electron proton transfer pathway. Kwon H, Basran J, Devos JM, Suardíaz R, van der Kamp MW, Mulholland AJ, Schrader TE, Ostermann A, Blakeley MP, Moody PCE, Raven EL. Proc Natl Acad Sci U S A 117 6484-6490 (2020)
  9. Direct Recording of Trans-Plasma Membrane Electron Currents Mediated by a Member of the Cytochrome b561 Family of Soybean. Picco C, Scholz-Starke J, Festa M, Costa A, Sparla F, Trost P, Carpaneto A. Plant Physiol 169 986-995 (2015)
  10. Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in Escherichia coli cell factories. Wu T, Ye L, Zhao D, Li S, Li Q, Zhang B, Bi C. 3 Biotech 8 269 (2018)
  11. Regulation by the quorum sensor from Vibrio indicates a receptor function for the membrane anchors of adenylate cyclases. Beltz S, Bassler J, Schultz JE. Elife 5 e13098 (2016)
  12. The cyanobacterial protoporphyrinogen oxidase HemJ is a new b-type heme protein functionally coupled with coproporphyrinogen III oxidase. Skotnicová P, Sobotka R, Shepherd M, Hájek J, Hrouzek P, Tichý M. J Biol Chem 293 12394-12404 (2018)
  13. AIR12, a b-type cytochrome of the plasma membrane of Arabidopsis thaliana is a negative regulator of resistance against Botrytis cinerea. Costa A, Barbaro MR, Sicilia F, Preger V, Krieger-Liszkay A, Sparla F, De Lorenzo G, Trost P. Plant Sci 233 32-43 (2015)
  14. Characterization of an l-Ascorbate Catabolic Pathway with Unprecedented Enzymatic Transformations. Stack TMM, Morrison KN, Dettmer TM, Wille B, Kim C, Joyce R, Jermain M, Naing YT, Bhatti K, Francisco BS, Carter MS, Gerlt JA. J Am Chem Soc 142 1657-1661 (2020)
  15. Direct measurements of ferric reductase activity of human 101F6 and its enhancement upon reconstitution into phospholipid bilayer nanodisc. El Behery M, Fujimura M, Kimura T, Tsubaki M. Biochem Biophys Rep 21 100730 (2020)
  16. Distribution and diversity of ROS-generating enzymes across the animal kingdom, with a focus on sponges (Porifera). Hewitt OH, Degnan SM. BMC Biol 20 212 (2022)
  17. Tuning of Hemes b Equilibrium Redox Potential Is Not Required for Cross-Membrane Electron Transfer. Pintscher S, Kuleta P, Cieluch E, Borek A, Sarewicz M, Osyczka A. J Biol Chem 291 6872-6881 (2016)
  18. Induced Resistance Mechanism of Novel Curcumin Analogs Bearing a Quinazoline Moiety to Plant Virus. Yin L, Gan X, Shi J, Zan N, Zhang A, Ren X, Li M, Xie D, Hu D, Song B. Int J Mol Sci 19 E4065 (2018)
  19. Interfaces Between Alpha-helical Integral Membrane Proteins: Characterization, Prediction, and Docking. Li B, Mendenhall J, Meiler J. Comput Struct Biotechnol J 17 699-711 (2019)
  20. Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency. Xie Q, Li S, Zhao D, Ye L, Li Q, Zhang X, Zhu L, Bi C. Microb Cell Fact 19 229 (2020)
  21. MtWRP1, a Novel Fabacean Specific Gene, Regulates Root Nodulation and Plant Growth in Medicago truncatula. Chen W, Chi Y, Zhang J, Bai B, Ji X, Shen Y. Genes (Basel) 13 193 (2022)
  22. Cellular production of a de novo membrane cytochrome. Hardy BJ, Martin Hermosilla A, Chinthapalli DK, Robinson CV, Anderson JLR, Curnow P. Proc Natl Acad Sci U S A 120 e2300137120 (2023)
  23. Comparison of insect and human cytochrome b561 proteins: Insights into candidate ferric reductases in insects. Holst JD, Murphy LG, Gorman MJ, Ragan EJ. PLoS One 18 e0291564 (2023)
  24. Electron current recordings in living cells. Trost P, Picco C, Scholz-Starke J, Festa M, Lagostena L, Costa A, Sparla F, Carpaneto A. Biophys Chem 229 57-61 (2017)
  25. Spectral and Redox Properties of a Recombinant Mouse Cytochrome b561 Protein Suggest Transmembrane Electron Transfer Function. Bérczi A, Márton Z, Laskay K, Tóth A, Rákhely G, Duzs Á, Sebők-Nagy K, Páli T, Zimányi L. Molecules 28 2261 (2023)


Quick links