› 4o81

X-ray diffraction
2.1Å resolution

SAICAR synthetase (Type-1) in complex with ADP AND AMP

Released:
DOI: 10.2210/pdb4o81/pdb
PDB entry 4o81 coloured by chain, front view.
PDB entry 4o81 coloured by chain, side view.
PDB entry 4o81 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Manjunath K, Jeyakanthan J, Sekar K

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-129650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.76 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57978 (Residues: 1-238; Coverage: 100%)
Gene names: PH0239, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CD 17 x CD
Ligand AMP 3 x AMP
Ligand BU1 2 x BU1
Ligand ADP 1 x ADP
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R3
Unit cell:
a: 95.14Å b: 95.14Å c: 148.251Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.255
Expression system: Escherichia coli

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