4oe1

X-ray diffraction
2.8Å resolution

Crystal structure of the pentatricopeptide repeat protein PPR10 (C256S/C430S/C449S) in complex with an 18-nt PSAJ rna element

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-110782 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pentatricopeptide repeat-containing protein 10, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 718 amino acids
Theoretical weight: 79.3 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: B8Y6I0 (Residues: 69-786; Coverage: 91%)
Gene names: PPR10, ZEAMMB73_Zm00001d036698
Sequence domains:
psaJ RNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 18 nucleotides
Theoretical weight: 5.6 KDa
Source organism: Zea mays
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43
Unit cell:
a: 83.318Å b: 83.318Å c: 225.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.263 0.278
Expression systems:
  • Escherichia coli
  • Not provided