› 4ohc

X-ray diffraction
1.85Å resolution

Crystal structure of orotate phosphoribosyltransferase (OPRTase) from Burkholderia cenocepacia

Released:
DOI: 10.2210/pdb4ohc/pdb
PDB entry 4ohc coloured by chain, front view.
PDB entry 4ohc coloured by chain, side view.
PDB entry 4ohc coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-109988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotate phosphoribosyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 236 amino acids
Theoretical weight: 26.56 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4E589 (Residues: 1-228; Coverage: 100%)
Gene names: BCAL0204, pyrE
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand EDO 7 x EDO
Ligand ACT 6 x ACT
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 76.31Å b: 82.97Å c: 83.1Å
α: 105.83° β: 104.27° γ: 117.36°
R-values:
R R work R free
0.158 0.156 0.197
Expression system: Escherichia coli

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