› 4ohr

X-ray diffraction
1.8Å resolution

Crystal structure of MilB from Streptomyces rimofaciens

Released:
DOI: 10.2210/pdb4ohr/pdb
PDB entry 4ohr coloured by chain, front view.
PDB entry 4ohr coloured by chain, side view.
PDB entry 4ohr coloured by chain, top view.
Source organism: Streptomyces rimofaciens
Primary publication:
Structure of the N-glycosidase MilB in complex with hydroxymethyl CMP reveals its Arg23 specifically recognizes the substrate and controls its entry.
Zhao G, Wu G, Zhang Y, Liu G, Han T, Deng Z, He X
OpenAccess logo Nucleic Acids Res 42 8115-24 (2014)
PMID: 24920828

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-110158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CMP/hydroxymethyl CMP hydrolase Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 20.53 KDa
Source organism: Streptomyces rimofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: B4Y381 (Residues: 1-170; Coverage: 100%)
Gene name: MilB
Sequence domains: Nucleoside 2-deoxyribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 46.294Å b: 102Å c: 81.956Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.216
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Nucleoside antibiotics: biosynthesis, regulation, and biotechnology.
Niu et al. (2015)
2 more

2 mentions without citation

Identification and characterization of enzymes involved in the biosynthesis of pyrimidine nucleoside antibiotics.
McErlean et al. (2021)
1 more