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The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15.

Figure 1
Tanley SW, Starkey LV, Lamplough L, Kaenket S, Helliwell JR
OpenAccess logo Acta Crystallogr F Struct Biol Commun 70 1132-4 (2014)
Related entries: 4owc, 4owe

Cited: 6 times
EuropePMC logo PMID: 25195880

Abstract

This study examines the binding and chemical stability of the platinum hexahalides K2PtCl6, K2PtBr6 and K2PtI6 when soaked into pre-grown hen egg-white lysozyme (HEWL) crystals as the protein host. Direct comparison of the iodo complex with the chloro and bromo complexes shows that the iodo complex is partly chemically transformed to a square-planar PtI3 complex bound to the N(δ) atom of His15, a chemical behaviour that is not exhibited by the chloro or bromo complexes. Each complex does, however, bind to HEWL in its octahedral form either at one site (PtI6) or at two sites (PtBr6 and PtCl6). As heavy-atom derivatives of a protein, the octahedral shape of the hexahalides could be helpful in cases of difficult-to-interpret electron-density maps as they would be recognisable 'objects'.

Articles - 4owh mentioned but not cited (1)

  1. The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15. Tanley SW, Starkey LV, Lamplough L, Kaenket S, Helliwell JR. Acta Crystallogr F Struct Biol Commun 70 1132-1134 (2014)


Reviews citing this publication (1)

  1. Trends in coordination of rhenium organometallic complexes in the Protein Data Bank. Brink A, Jacobs FJF, Helliwell JR. IUCrJ 9 180-193 (2022)

Articles citing this publication (4)

  1. Orthorhombic lysozyme crystallization at acidic pH values driven by phosphate binding. Plaza-Garrido M, Salinas-Garcia MC, Camara-Artigas A. Acta Crystallogr D Struct Biol 74 480-489 (2018)
  2. Reaction with Proteins of a Five-Coordinate Platinum(II) Compound. Ferraro G, Marzo T, Cucciolito ME, Ruffo F, Messori L, Merlino A. Int J Mol Sci 20 E520 (2019)
  3. Impact of Hydrophobic Chains in Five-Coordinate Glucoconjugate Pt(II) Anticancer Agents. Annunziata A, Imbimbo P, Cucciolito ME, Ferraro G, Langellotti V, Marano A, Melchiorre M, Tito G, Trifuoggi M, Monti DM, Merlino A, Ruffo F. Int J Mol Sci 24 2369 (2023)
  4. X-ray diffraction in temporally and spatially resolved biomolecular science. Helliwell JR, Brink A, Kaenket S, Starkey VL, Tanley SW. Faraday Discuss 177 429-441 (2015)


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