› 4owu

X-ray diffraction
1.89Å resolution

Anthranilate phosphoribosyl transferase from Mycobacterium tuberculosis in complex with 5-methylanthranilate, PRPP and Magnesium

Released:
DOI: 10.2210/pdb4owu/pdb
PDB entry 4owu coloured by chain, front view.
PDB entry 4owu coloured by chain, side view.
PDB entry 4owu coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Alternative substrates reveal catalytic cycle and key binding events in the reaction catalysed by anthranilate phosphoribosyltransferase from Mycobacterium tuberculosis.
Cookson TV, Castell A, Bulloch EM, Evans GL, Short FL, Baker EN, Lott JS, Parker EJ
Biochem J 461 87-98 (2014)
PMID: 24712732

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 378 amino acids
Theoretical weight: 38.85 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFX5 (Residues: 1-370; Coverage: 100%)
Gene names: MTCY190.03c, Rv2192c, trpD
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand PRP 2 x PRP
Ligand 5M0 2 x 5M0
Ligand GOL 3 x GOL
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 79.486Å b: 91.792Å c: 120.801Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
2 more

2 mentions without citation

The tryptophan biosynthetic pathway is essential for Mycobacterium tuberculosis to cause disease.
Lott JS. (2020)
1 more