› 4oy2

X-ray diffraction
2.9Å resolution

Crystal structure of TAF1-TAF7, a TFIID subcomplex

Released:
DOI: 10.2210/pdb4oy2/pdb
PDB entry 4oy2 coloured by chain, front view.
PDB entry 4oy2 coloured by chain, side view.
PDB entry 4oy2 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural and functional insight into TAF1-TAF7, a subcomplex of transcription factor II D.
Bhattacharya S, Lou X, Hwang P, Rajashankar KR, Wang X, Gustafsson JÅ, Fletterick RJ, Jacobson RH, Webb P
Proc Natl Acad Sci U S A 111 9103-8 (2014)
PMID: 24927529

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155505 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription initiation factor TFIID subunit 1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 506 amino acids
Theoretical weight: 58.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P46677 (Residues: 455-960; Coverage: 48%)
Gene names: G9374, TAF1, TAF130, TAF145, YGR274C
Sequence domains: Protein of unknown function (DUF3591)
Transcription initiation factor TFIID subunit 7 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 235 amino acids
Theoretical weight: 27.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q05021 (Residues: 86-320; Coverage: 40%)
Gene names: TAF67, TAF7, YM9959.09C, YMR227C
Sequence domains: TAFII55 protein conserved region

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand TRS 1 x TRS
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 122.215Å b: 123.66Å c: 229.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.243
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Structural basis of transcription initiation by RNA polymerase II.
Sainsbury et al. (2015)
6 more

2 mentions without citation

Zooming in on Transcription Preinitiation.
Gupta et al. (2016)
1 more