› 4oy4

X-ray diffraction
2.03Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144019 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green to red photoconvertible GFP-like protein EosFP; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 47.29 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP29 (Residues: 3-147; Coverage: 97%)
  • Canonical: Q5S6Z9 (Residues: 2-135, 148-161; Coverage: 66%)
  • Canonical: P11799 (Residues: 1719-1725, 1745-1749; Coverage: 1%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1, Mylk
Sequence domains:
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand CR8 1 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 68.67Å b: 68.67Å c: 172.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.235
Expression system: Escherichia coli

Quick links

Citations

89 review citations

Genetically encoded indicators of neuronal activity.
Lin et al. (2016)
88 more

1 mention without citation

Improved methods for marking active neuron populations.
Moeyaert et al. (2018)