› 4p3l

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM CHROMOHALOBACTER SALEXIGENS DSM 3043 (Csal_2479), TARGET EFI-510085, WITH BOUND GLUCURONATE, SPG P6122

Released:
DOI: 10.2210/pdb4p3l/pdb
Model geometry
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PDB entry 4p3l coloured by chain, front view.
PDB entry 4p3l coloured by chain, side view.
PDB entry 4p3l coloured by chain, top view.
Source organism: Chromohalobacter salexigens DSM 3043
Primary publication:
Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting MW, Al-Obaidi N, Zhao S, San Francisco B, Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ, Osterman AL, Cronan JE, Jacobson MP, Gerlt JA, Almo SC
OpenAccess logo Biochemistry 54 909-31 (2015)
PMID: 25540822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173015 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute-binding protein Csal_2479 Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 37 KDa
Source organism: Chromohalobacter salexigens DSM 3043
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1QUN2 (Residues: 34-336; Coverage: 100%)
Gene names: Csal_2479, dctP
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand BDP 1 x BDP
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P6122
Unit cell:
a: 160.657Å b: 160.657Å c: 58.117Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.144 0.142 0.167
Expression system: Escherichia coli BL21(DE3)

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Citations

14 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
13 more

1 mention without citation

Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting et al. (2015)

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