› 4p7x

X-ray diffraction
1.3Å resolution

Function and Biology Details

Reaction catalysed: 
L-proline + 2-oxoglutarate + O(2) = cis-4-hydroxy-L-proline + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-proline cis-4-hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.59 KDa
Source organism: Mesorhizobium japonicum MAFF 303099
Expression system: Escherichia coli
UniProt:
  • Canonical: Q989T9 (Residues: 2-280; Coverage: 100%)
Gene name: mlr6283
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand CO 1 x CO
Ligand AKG 1 x AKG
Ligand YCP 1 x YCP
Ligand SO4 4 x SO4
Ligand CXS 2 x CXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2221
Unit cell:
a: 112.68Å b: 123.395Å c: 56.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.135 0.162
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metabolic engineering strategy for synthetizing trans-4-hydroxy-L-proline in microorganisms.
Zhang et al. (2021)
1 more

3 mentions without citation

Echinocandins: structural diversity, biosynthesis, and development of antimycotics.
Hüttel W. (2021)
2 more