› 4pbq

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM HAEMOPHILUS INFLUENZAE RdAW (HICG_00826, TARGET EFI-510123) WITH BOUND L-GULONATE

Released:
DOI: 10.2210/pdb4pbq/pdb
Model geometry
Fit model/data
PDB entry 4pbq coloured by chain, front view.
PDB entry 4pbq coloured by chain, side view.
PDB entry 4pbq coloured by chain, top view.
Source organism: Haemophilus influenzae RdAW
Primary publication:
Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting MW, Al-Obaidi N, Zhao S, San Francisco B, Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ, Osterman AL, Cronan JE, Jacobson MP, Gerlt JA, Almo SC
OpenAccess logo Biochemistry 54 909-31 (2015)
PMID: 25540822

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative TRAP PERIPLASMIC SOLUTE BINDING PROTEIN Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 327 amino acids
Theoretical weight: 38.28 KDa
Source organism: Haemophilus influenzae RdAW
Expression system: Escherichia coli BL21(DE3)
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

4 bound ligands:
Ligand 2UF 3 x 2UF
Ligand SO4 4 x SO4
Ligand MES 1 x MES
Ligand PEG 3 x PEG
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 133.449Å b: 76.537Å c: 112.977Å
α: 90° β: 114.01° γ: 90°
R-values:
R R work R free
0.149 0.147 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
13 more

2 mentions without citation

Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting et al. (2015)
1 more