› 4pef

X-ray diffraction
1.96Å resolution

Dbr1 in complex with sulfate

Released:
DOI: 10.2210/pdb4pef/pdb
PDB entry 4pef coloured by chain, front view.
PDB entry 4pef coloured by chain, side view.
PDB entry 4pef coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Structural basis of lariat RNA recognition by the intron debranching enzyme Dbr1.
Montemayor EJ, Katolik A, Clark NE, Taylor AB, Schuermann JP, Combs DJ, Johnsson R, Holloway SP, Stevens SW, Damha MJ, Hart PJ
OpenAccess logo Nucleic Acids Res 42 10845-55 (2014)
PMID: 25123664

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-504043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lariat debranching enzyme Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 360 amino acids
Theoretical weight: 41.91 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4M1P9 (Residues: 1-354; Coverage: 100%)
Gene names: DBR1, EHI_062730
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MN 5 x MN
Ligand SO4 10 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 73.217Å b: 141.694Å c: 213.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.232
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Influenza Pathogenesis: The Effect of Host Factors on Severity of Disease.
Gounder et al. (2019)
6 more

3 mentions without citation

In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity.
Pires et al. (2016)
2 more